Anti-MARCKS Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P29966

Core Product Specifications and Parameters

Biological Background: MARCKS Function and Localization

• MARCKS (Myristoylated alanine-rich C-kinase substrate) is a membrane-associated protein that plays a key role in modulating the actin cytoskeleton, regulating processes such as chemotaxis, cell motility, adhesion, phagocytosis, and exocytosis. Related references: PMID:23704996, PMID:36009319
• Acts as a phosphatidylinositol 4,5-bisphosphate (PIP2) sequestering protein at lipid rafts in the plasma membrane; this sequestration is reversed by protein kinase C, leading to exocytosis inhibition. Related references: PMID:23704996
• During inflammation, MARCKS promotes migration and adhesion of inflammatory cells and enhances secretion of cytokines such as TNF, particularly in macrophages. Related references: PMID:37949888
• Essential for bacteria-induced intracellular reactive oxygen species (ROS) formation in monocytic cells.
• Contributes to neurite initiation and outgrowth by interacting with CDC42, a regulator of cell shape and cytoskeletal dynamics (By similarity).
• Plays a role in axon development by mediating docking and fusion of RAB10-positive vesicles with the plasma membrane (By similarity).
• Post-translationally modified by myristoylation and phosphorylation; also acetylated, and contains actin- and calmodulin-binding domains.
• Detected in spermatozoa, suggesting a role in reproductive biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human MARCKS. Consider potential cross-reactivity with other species or isoforms due to sequence conservation.
• For western blot (WB), the predicted molecular weight is ~32 kDa, but post-translational modifications may cause band shifts. Validate in relevant cell lines.
• Suitable for immunofluorescence and ELISA, but optimal dilutions should be determined experimentally.
• Due to its membrane and cytoskeletal association, consider cellular fractionation or specific extraction conditions to enrich the target protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop platform supplying IVD raw materials to diagnostic manufacturers and research laboratories, covering every stage from concept to clinic. This high-quality MARCKS polyclonal antibody supports research in cell signaling, cytoskeletal dynamics, and immunology. Trust CamelBio to provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your development pipelines.