Anti-MARCH9 Polyclonal Antibody for WB, ELISA - Q86YJ5

Core Product Specifications and Parameters

Biological Background: MARCH9 (MARCHF9) Function and Localization

• MARCH9 (also designated MARCHF9, RNF179, or MARCH-IX) is a member of the membrane-associated RING-CH (MARCH) family of E3 ubiquitin ligases, featuring a characteristic RING-variant zinc-finger domain.
• The primary function of MARCH9 is to ubiquitinate key immune receptors, including MHC-I, CD4, and ICAM1, thereby promoting their endocytosis and subsequent sorting to lysosomes via multivesicular bodies.
• As an E3 ubiquitin ligase, MARCH9 accepts ubiquitin from E2 conjugating enzymes and directly transfers it to substrate proteins, playing an essential role in the ubiquitin-proteasome and endo-lysosomal degradation pathways.
• Subcellularly, MARCH9 localizes to the membranes of the Golgi apparatus and lysosomes, consistent with its involvement in protein trafficking and degradation.
• The protein is ubiquitously expressed across human tissues, suggesting a broad regulatory role in immune surveillance and cellular homeostasis.
• MARCH9 contains a transmembrane helix and a zinc-finger domain, and the encoding gene undergoes alternative splicing, potentially yielding multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 277-346 near the C-terminus of human MARCH9; consider verifying reactivity with specific isoforms if alternative splicing is a concern in your experimental system.
• For Western blot analysis, a band of approximately 38 kDa is expected; however, post-translational modifications or protein interactions may cause migration shifts—validate with positive and negative controls.
• This polyclonal antibody is suitable for ELISA applications; consider using recombinant MARCH9 protein or blocking peptides to confirm binding specificity and optimize assay conditions.
• Cross-reactivity with other MARCH family members or RING-finger proteins may occur due to domain conservation; empirical testing in your sample type is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, providing one-stop access to premium antibodies like this anti-MARCH9 polyclonal antibody for immune receptor trafficking studies. Our supply network delivers validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials to streamline assay development. Whether you require standard catalog products or custom sourcing for rare targets, CamelBio accelerates your path from research to clinically validated diagnostics.