Anti-MARCH8 Polyclonal Antibody for WB and ELISA - Q5T0T0

Core Product Specifications and Parameters

Biological Background: MARCH8 (MARCHF8) Function and Localization

• MARCH8 (also known as c-MIR, RNF178, MARCH-VIII) is an E3 ubiquitin-protein ligase containing a RING finger domain that functions as a ubiquitin transferase in various immune pathways.
• It mediates ubiquitination of CD86 and MHC class II proteins (HLA-DR alpha and beta), promoting their endocytosis and lysosomal degradation, thus downregulating antigen presentation (PubMed:19117940, PubMed:19566897). Related references: PMID:19117940 PMID:19566897
• Exhibits broad antiviral activity by targeting cytoplasmic lysine residues of viral envelope glycoproteins (e.g., HIV-1 Env, Ebola GP, influenza M2) for lysosomal degradation, blocking viral entry and particle release (PubMed:32934085, PubMed:34285233, PubMed:35019698). Related references: PMID:32934085 PMID:34285233 PMID:35019698
• Also regulates immune tolerance by promoting proteasomal degradation of PD-L1/CD274 via ubiquitination (PubMed:34183449) and inhibits cGAS-mediated innate immunity through 'Lys-63'-linked polyubiquitination (PubMed:35503863). Related references: PMID:34183449 PMID:35503863
• Negatively modulates IL7-mediated T-cell homeostasis by mediating 'Lys-27'-linked polyubiquitination and lysosomal degradation of IL7R (PubMed:39311660). Related references: PMID:39311660
• Subcellular localization includes Golgi apparatus, endoplasmic reticulum, cytoplasmic vesicle, lysosome, and early endosome membranes; broadly expressed, with confirmed presence in immature dendritic cells.
• Keywords: Metal-binding, zinc-finger, phosphoprotein, alternative splicing, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen (aa 232-291) lies within the cytoplasmic region of MARCH8; thus, the antibody may detect both full-length and truncated isoforms. Consider epitope mapping for application-specific validation.
• For Western blotting, test human, mouse, and rat cell lysates, as MARCH8 is broadly expressed. Optimize loading controls and antibody dilutions empirically.
• In ELISA applications, use recombinant MARCH8 protein or cell lysates as coating antigens; polyclonal antibodies may require careful titration to minimize background.
• Given its role in viral restriction, this antibody may be useful in studying virus-host interactions, but validate in appropriate infection models and note potential cross-reactivity with other MARCH family members.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we are your one-stop bridge for IVD raw material sourcing, offering a comprehensive portfolio of validated antibodies, antibody pairs, and ancillary reagents. This MARCH8 polyclonal antibody supports research into innate and adaptive immunity, viral evasion, and immune checkpoint regulation. Whether you need bulk quantities, custom development, or rare target sourcing, CamelBio provides end-to-end support from concept to clinic.