Anti-MAPT Polyclonal Antibody for WB, IF/ICC, ELISA - P10637

Core Product Specifications and Parameters

Biological Background: MAPT Function and Localization

• MAPT (Microtubule-associated protein tau), also known as Tau or PHF-tau, is a neuronal microtubule-associated protein encoded by the Mapt gene in mouse (Gene ID: 17762) and MAPT in human (Gene ID: 4137).
• Tau promotes microtubule assembly and stability, and is thought to be involved in establishing and maintaining neuronal polarity. It binds axonal microtubules via its C-terminus and neural plasma membrane components via its N-terminus, suggesting a linker function.
• Multiple isoforms are generated by alternative splicing. Shorter isoforms impart cytoskeletal plasticity while longer isoforms promote stabilization. Isoform PNS-tau is expressed in the peripheral nervous system, with others in the CNS.
• Subcellular localization includes cytoplasm, cytosol, cell membrane, cytoskeleton, axonal and dendritic projections, and it can also be secreted.
• Tau is subject to extensive post-translational modifications including phosphorylation, acetylation, methylation, glycation, disulfide bond formation, and ubiquitination, as reflected in UniProt keywords.
• In neurons, tau is predominantly expressed, but low-level expression can be detected in liver and kidney.
• The protein is highly relevant to neurodegenerative diseases; its hyperphosphorylated forms aggregate into paired helical filaments (PHF-tau) and neurofibrillary tangles, hallmarks of Alzheimer's disease and related tauopathies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 3-214) of mouse MAPT, which may generate antibodies recognizing multiple isoforms spanning the predicted molecular weights of ~35-38 kDa, 44 kDa, and 76 kDa. When performing Western blotting, expect bands within these ranges depending on the isoform expressed in your sample.
• For immunofluorescence/immunocytochemistry (IF/ICC), consider optimizing fixation and permeabilization conditions, as tau localizes to multiple subcellular structures including microtubules, cytoplasmic compartments, and the plasma membrane. Co-staining with a neuronal marker may help verify specificity.
• As a polyclonal antibody, batch-to-batch consistency should be validated in the relevant experimental system. Users are advised to titrate the antibody concentration for ELISA to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, supporting diagnostic manufacturers and research laboratories with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement. For tau-targeted research and assay development, we offer ready-to-ship polyclonal antibodies like this anti-MAPT antibody to streamline your workflows from concept to clinic.