Anti-MAPKAPK-2/MK2 Rabbit Polyclonal Antibody for WB and ELISA - P49137

Core Product Specifications and Parameters

Biological Background: MAPKAPK-2/MK2 Function and Localization

• MAPKAPK-2 (MK2) is a serine/threonine-protein kinase encoded by the MAPKAPK2 gene. Alternative splicing produces multiple isoforms.
• It is a downstream effector of p38 MAP kinase, activated by stress stimuli, and phosphorylates substrates to regulate diverse processes including cytokine production, endocytosis, cytoskeletal reorganization, cell migration, and cell cycle control.
• Subcellular localization: predominantly cytoplasmic but can translocate to the nucleus under certain conditions.
• Key roles in post-transcriptional regulation: phosphorylates ARE-binding proteins (ELAVL1, HNRNPA0, PABPC1, TTP/ZFP36) to modulate stability and translation of TNF and IL6 mRNAs.
• In DNA damage response, relocalizes from nucleus to cytoplasm, phosphorylating HNRNPA0 and PARN to stabilize GADD45A mRNA, contributing to G2/M checkpoint maintenance.
• Phosphorylates HSF1, promoting interaction with HSP90 and inhibiting homotrimerization and transcriptional activity (PubMed:16278218). Related references: PMID:16278218
• Phosphorylates CEP131 under UV stress, enhancing 14-3-3 binding and suppressing centriolar satellite formation (PubMed:26616734). Related references: PMID:26616734
• Expressed ubiquitously across human tissues. Belongs to the protein kinase superfamily, with features including ATP-binding, phosphoprotein status, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 300-400 of human MAPKAPK-2 (NP_116584.2). When using this polyclonal antibody, consider validating specificity in your model system, as the exact epitope(s) recognized may influence cross-reactivity with isoforms or orthologs.
• For Western blotting, a band of approximately 46 kDa is expected. Optimize blocking and antibody dilution conditions using positive and negative control lysates (e.g., stimulated vs. unstimulated cells).
• For ELISA applications, ensure appropriate coating and detection conditions; consider pairing with a MAPKAPK-2-specific capture antibody if developing a sandwich assay.
• Given the antibody's cross-reactivity with rat, it may be suitable for studies in rodent models; however, verify performance in the relevant tissue or cell type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive platform for diagnostic manufacturers and research labs to source high-quality IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This anti-MAPKAPK-2/MK2 polyclonal antibody supports research into stress signaling and cytokine biology, and is part of our extensive catalog covering kinases and cell signaling targets. Beyond individual reagents, we offer tailored sourcing solutions and technical consultation to accelerate your assay development from concept to clinical application.