Anti-MAPKAPK-2/MK2 Monoclonal Antibody for WB, ELISA - P49137

Core Product Specifications and Parameters

Biological Background: MAPKAPK-2/MK2 Function and Localization

• MAPKAPK-2/MK2 is a stress-activated serine/threonine-protein kinase involved in multiple cellular processes, including cytokine production, cytoskeleton reorganization, cell migration, cell cycle control, chromatin remodeling, DNA damage response, and transcriptional regulation.
• Upon stress, it is phosphorylated and activated by p38-alpha/MAPK14, leading to phosphorylation of downstream substrates on serine residues within the consensus sequence Hyd-X-R-X(2)-S.
• It phosphorylates HSF1, promoting interaction with HSP90 and inhibiting HSF1 homotrimerization, DNA-binding, and transactivation activities, thereby modulating the heat shock response. Related references: PMID:16278218
• In response to UV irradiation, it phosphorylates CEP131, which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites. Related references: PMID:26616734
• It plays a key role in post-transcriptional regulation of inflammatory cytokines such as TNF and IL6 by phosphorylating AU-rich element (ARE)-binding proteins (e.g., TTP/ZFP36, ELAVL1, PABPC1), thus modulating mRNA stability and translation.
• MAPKAPK-2/MK2 contributes to the late G2/M DNA damage checkpoint by relocalizing from nucleus to cytoplasm after DNA damage and phosphorylating HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA.
• It mediates phosphorylation of HSP27/HSPB1 in response to stress, causing dissociation of large sHSP oligomers and impairing chaperone activity.
• Subcellular localization is predominantly cytoplasmic and nuclear, and it is ubiquitously expressed in all tissues examined.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300–400 of human MAPKAPK-2/MK2, targeting an internal region of the protein; ensure that your detection system is compatible with this epitope location.
• For Western blot, a band around 46 kDa is expected; consider using positive control lysates from human, mouse, or rat cells and include both stressed and non-stressed samples to verify activation-dependent detection.
• When using this antibody in ELISA, optimize coating conditions and validate with recombinant MAPKAPK-2 protein or cell extracts from appropriate species to confirm specificity.
• Depending on the assay context, be aware that post-translational modifications (e.g., phosphorylation) could affect antibody binding; consider phosphatase inhibitor treatments if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for sourcing IVD raw materials, supporting your assay development from concept to clinic. For MAPKAPK-2/MK2 and other stress-kinase targets, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expert assistance in rare target sourcing. Whether you are developing diagnostic kits for inflammatory markers or DNA damage response panels, CamelBio streamlines procurement so you can focus on advancing your research and clinical applications.