Anti-MAPKAP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BPZ7

Core Product Specifications and Parameters

Biological Background: Target of rapamycin complex 2 subunit MAPKAP1 Function and Localization

• MAPKAP1 (also known as SIN1) is a core component of the mechanistic target of rapamycin complex 2 (mTORC2), which integrates growth factor signals to control cell proliferation, cytoskeletal organization, and anabolic metabolism. Related references: PMID:15467718 PMID:16919458 PMID:16962653
• Within mTORC2, MAPKAP1 functions as a substrate adaptor, recognizing and presenting AGC kinase family members (including AKT, PKC, and SGK1) for phosphorylation by MTOR on critical regulatory sites (e.g., AKT1 Ser-473, SGK1 Ser-422, PRKCA Ser-657). Related references: PMID:16919458 PMID:16962653 PMID:21806543 PMID:28264193 PMID:35926713 PMID:30837283
• Beyond mTORC2, MAPKAP1 exerts distinct functions: it inhibits MAP3K2 dimerization/autophosphorylation, suppresses HRAS and KRAS signaling, enhances osmotic stress-induced ATF2 activation, and regulates cilia length via CCDC28B. Related references: PMID:15988011 PMID:17303383 PMID:34380736 PMID:35522713 PMID:17054722 PMID:23727834
• The gene produces multiple isoforms through alternative splicing; notably, isoform 4 does not incorporate into mTORC2, indicating isoform-specific functional divergence.
• MAPKAP1 localizes to diverse cellular compartments, including the plasma membrane, endoplasmic reticulum, endosomes, lysosomes, Golgi apparatus, mitochondria, cytoplasm, and nucleus, reflecting its multifaceted signaling roles.
• Ubiquitously expressed with highest levels in heart and skeletal muscle; post-translationally regulated by acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 360–522 of human MAPKAP1 (isoform 1), a region well-conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blotting, a band of approximately 59 kDa is expected; lysates from tissues with high MAPKAP1 expression (e.g., heart, skeletal muscle) or cell lines stimulated with serum may serve as positive controls.
• In immunohistochemistry (IHC-P), validation on formalin-fixed paraffin-embedded (FFPE) sections is recommended; consider antigen retrieval conditions and appropriate controls to confirm specificity.
• Note that polyclonal antibodies may detect multiple isoforms; evaluate the isoform(s) expressed in your experimental system and verify band patterns with knockdown or knockout controls.
• For ELISA, the antibody can be used as a capture or detection reagent; optimal working concentrations should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, providing validated antibodies, optimized polyclonal and monoclonal preparations, and bulk ancillary reagents. This anti-MAPKAP1 polyclonal antibody is aimed at researchers investigating mTOR signaling, cancer biology, and metabolic disorders. Leverage our one-stop supply chain for rare-target raw materials and technical support to accelerate your assay development from concept to clinic.