Anti-MAPK8 Rabbit Polyclonal Antibody for WB, ELISA - P45983

Core Product Specifications and Parameters

Biological Background: MAPK8 Function and Localization

• MAPK8 (JNK1/SAPK1c) is a stress-activated serine/threonine kinase that is central to the SAP/JNK signaling cascade, responding to cytokines and environmental stress. Related references: PMID:28943315
• Upon activation by dual phosphorylation from MAP2K4/MKK4 and MAP2K7/MKK7, MAPK8 phosphorylates transcription factors JUN, JDP2, and ATF2 to regulate AP-1 transcriptional activity. Related references: PMID:18307971
• In the nucleus, MAPK8 phosphorylates the replication licensing factor CDT1, disrupting its interaction with HBO1 and inhibiting replication origin acetylation, thus controlling DNA replication initiation. Related references: PMID:21856198
• MAPK8 drives apoptosis by phosphorylating tumor suppressors p53/TP53 and YAP1, and modulates mitochondrial cell death through phosphorylation of BAD and BCL2, linking it to autophagy and cell survival. Related references: PMID:21364637, PMID:21095239, PMID:18570871
• It also plays a role in circadian rhythm regulation by directly phosphorylating the CLOCK-BMAL1 heterodimer. Related references: PMID:22441692
• MAPK8 is widely distributed in the cytoplasm and nucleus, and is also detected at synaptic sites.
• Alternative splicing generates multiple isoforms (alpha-1, alpha-2, beta-1, beta-2) that differentially bind c-Jun and ATF2 but show comparable phosphorylation efficiency.
• Additionally, MAPK8 targets heat shock factors (HSF1, HSF4), deacetylase SIRT1, transcription factor ELK1, and E3 ligase ITCH, reflecting its diverse regulatory functions. Related references: PMID:10747973, PMID:16581800, PMID:17296730, PMID:20027304

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human MAPK8, and the resulting polyclonal antibody is expected to recognize multiple isoforms and potentially cross-react with mouse and rat MAPK8, as indicated by the supplier.
• For Western blotting, a band at approximately 48 kDa should be observed; pre-validation in the cell or tissue lysate of interest is advisable.
• This antibody has been demonstrated in ELISA; optimization of coating antigen and detection antibody dilutions may be required for quantitative applications.
• When using for mouse or rat samples, confirm specificity due to the high conservation of the N-terminus across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-quality IVD antibody raw materials, including the MAPK8 rabbit polyclonal antibody, supporting research into stress kinase signaling and oncology. Our one-stop sourcing platform offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom rare-target development to accelerate your diagnostic and research workflows.