Anti-MAPK13 Rabbit Polyclonal Antibody for WB and ELISA - O15264

Core Product Specifications and Parameters

Biological Background: MAPK13 Function and Localization

• MAPK13, also known as SAPK4, PRKM13, and p38δ, is a serine/threonine kinase and a member of the p38 mitogen-activated protein kinase (MAPK) family.
• It functions as a critical component of the MAPK signaling cascade, activated by extracellular stimuli such as pro-inflammatory cytokines, physical stress, and UV irradiation, leading to phosphorylation of downstream targets like transcription factors ELK1 and ATF2.
• p38 kinases have broad substrate specificity, with an estimated 200–300 substrates each; MAPK13 specifically regulates protein translation via EEF2K phosphorylation, cytoskeletal remodeling through MAPT and STMN1, and UV-induced gene expression (e.g., CXCL14).
• It plays a role in epidermal keratinocyte differentiation, apoptosis, and skin tumor development, and phosphorylates MYB to trigger its proteasome-dependent degradation.
• In pancreatic beta cells, MAPK13 phosphorylates and down-regulates PRKD1, contributing to the regulation of insulin secretion.
• Tissue expression is notable in testes, pancreas, small intestine, lung, kidney, and is abundant in macrophages, with presence also in neutrophils, CD4+ T-cells, and endothelial cells.
• The protein undergoes alternative splicing and phosphorylation, consistent with its regulation in stress responses and cell cycle control.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 1-365 of human MAPK13 (NP_002745.1), which covers the full-length sequence. This design is likely to produce antibodies recognizing multiple epitopes, suitable for detecting both endogenous and overexpressed protein.
• For Western blot, the predicted molecular weight is 42 kDa; consider running appropriate positive and negative controls (e.g., cell lines with known MAPK13 expression) and validate with siRNA knockdown if possible.
• In ELISA, the polyclonal nature can provide signal amplification; optimizing coating antigen concentration and antibody dilution is recommended for best signal-to-noise ratio.
• Cross-reactivity has been reported with mouse and rat samples; however, it is advisable to verify performance in your specific experimental system due to potential sequence differences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for the MAP kinase signaling and stress response research areas. We support manufacturers and labs from concept to clinic, providing reliable anti-MAPK13 antibodies and related products to accelerate your diagnostic and therapeutic development. Our supply capabilities include rare target raw-material sourcing, ensuring research continuity.