Anti-MAPK13 Polyclonal Antibody for WB, ELISA - O15264

Core Product Specifications and Parameters

Biological Background: MAPK13 Function and Localization

• MAPK13, also known as p38 delta or stress-activated protein kinase 4 (SAPK4), is a member of the p38 MAP kinase family and is encoded by the MAPK13 gene (Gene ID: 5603).
• As a serine/threonine kinase, MAPK13 plays a critical role in the MAP kinase signal transduction pathway, mediating cellular responses to pro-inflammatory cytokines and physical stress by phosphorylating transcription factors such as ELK1 and ATF2.
• MAPK13 regulates protein translation by phosphorylating and inactivating EEF2K, and is involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1.
• This kinase mediates UV irradiation-induced up-regulation of CXCL14 gene expression and plays a key role in epidermal keratinocyte differentiation, apoptosis, and skin tumor development.
• Under stress conditions, MAPK13 phosphorylates the transcriptional activator MYB, promoting its proteasome-dependent degradation.
• In pancreatic beta cells, MAPK13 phosphorylates and down-regulates PRKD1, contributing to the regulation of insulin secretion.
• MAPK13 is expressed in testes, pancreas, small intestine, lung, kidney, and is abundant in macrophages, neutrophils, CD4+ T-cells, and endothelial cells.
• Keywords associated with MAPK13 include alternative splicing, ATP-binding, cell cycle, kinase, stress response, and transcription regulation, reflecting its multifunctional roles.

Experimental Guidance and Technical Tips

• Based on the immunogen region (aa 300–400), the antibody is expected to recognize the target protein in Western blotting at approximately 42 kDa.
• For WB, consider using lysates from cell lines or tissues with confirmed MAPK13 expression (e.g., macrophages, pancreatic beta cells, or lung tissue).
• In ELISA assays, appropriate pairing and dilution should be empirically determined; the polyclonal nature offers broad epitope recognition.
• Cross-reactivity with mouse and rat MAPK13 suggests potential utility across species, but validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive one-stop platform for IVD raw-material procurement, including validated antibody pairs, high-affinity monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise in MAPK pathway targets ensures reliable sourcing for immunoassay development and stress-response research. Contact us to streamline your supply chain and accelerate progress from concept to clinic.