Anti-MAP4K5 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q9Y4K4

Core Product Specifications and Parameters

Biological Background: MAP4K5 Function and Localization

• Mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), also known as kinase homologous to SPS1/STE20 (KHS), is a serine/threonine-protein kinase belonging to the STE20 family.
• In response to environmental stress, MAP4K5 acts upstream of the JUN N-terminal pathway, implicating it in stress signaling and MAPK cascade regulation.
• The protein localizes to the cytoplasm, where it participates in cytoplasmic signaling networks.
• MAP4K5 is ubiquitously expressed, with particularly high levels in the ovary, testis, and prostate, suggesting tissue-specific roles.
• Key molecular features include ATP-binding, nucleotide-binding, and phosphorylation sites essential for catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 300–480) spans a region outside the core kinase domain, potentially minimizing cross‑reactivity with closely related kinases.
• Recommended for use in IF/ICC and ELISA; for other applications such as WB or IHC, validation in the appropriate sample matrix is advised.
• Cross‑reactivity with mouse and rat enables orthologous studies; consider species‑specific positive controls.
• Based on expression profiles, ovary, testis, or prostate lysates may serve as suitable positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-MAP4K5 polyclonal antibody supports investigations into stress‑activated kinase signaling, and complements our range of rare‑target and custom‑sourced reagents for preclinical and translational applications.