Anti-MAP4K2 Rabbit Polyclonal Antibody for WB & ELISA - Q12851

Core Product Specifications and Parameters

Biological Background: MAP4K2 Function and Localization

• MAP4K2 (also known as GCK, RAB8IP, BL44) is a serine/threonine-protein kinase that acts as a key MAP4K in the JNK and p38 MAPK signaling cascades.
• It is essential for innate immunity, being required for efficient JNK activation by TRAF6-dependent stimuli such as LPS, peptidoglycan, poly(I:C), and bacterial flagellin, and to a lesser extent by IL-1 and CD40 engagement.
• MAP4K2 functions in the Hippo signaling pathway by phosphorylating and activating LATS1/2, contributing to organ size control and tumor suppression. Related references: PMID:26437443
• The protein localizes to the cytoplasm, basolateral cell membrane, and Golgi apparatus membrane.
• Highly expressed in germinal center B-cells, with additional expression in lung, brain, and placenta.
• Involved in stress response, innate immunity, and possibly vesicle targeting or fusion; post-translational modifications include phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• This antibody, raised against a C-terminal fragment (aa 551-820), is suitable for Western blot detection of MAP4K2 (~92 kDa). Validate in the relevant sample system, considering tissue-specific expression patterns.
• For ELISA applications, the recombinant immunogen can serve as a positive control; optimize coating and detection conditions empirically.
• Cross-reactivity with mouse and rat MAP4K2 is indicated, but verify sequence homology in the targeted region to ensure consistent reactivity across species.

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