Anti-MAP3K5 Polyclonal Antibody for WB, ELISA - Q99683

Core Product Specifications and Parameters

Biological Background: MAP3K5 Function and Localization

• Serine/threonine kinase and essential component of the MAP kinase signal transduction pathway, mediating cellular responses to environmental changes, including differentiation, survival, and apoptosis through mitochondria-dependent caspase activation.
• Acts as an upstream activator of the MKK/JNK and p38 MAPK cascades by phosphorylating and activating MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6, and MAP2K7/MKK7, which then activate p38 MAPKs and JNKs to control AP-1 transcription factors.
• Plays a crucial role in the innate immune response and host defense against pathogens; mediates signaling triggered by stressors like oxidative stress, TNF, or LPS, linking receptor-mediated inflammatory signals to downstream kinase activation.
• Localizes to both the cytoplasm and the endoplasmic reticulum, with abundant expression observed in heart and pancreas tissues.
• Undergoes post-translational modifications including phosphorylation, methylation, and ubiquitin-like conjugation; contains an ATP-binding domain, coiled-coil region, and requires magnesium as a cofactor.
• Multiple isoforms are produced by alternative splicing, contributing to functional diversity and regulatory complexity in stress and immune signaling.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using whole-cell lysates from heart or pancreas tissues, where MAP3K5 is abundantly expressed, and optimize antibody dilution to achieve clear signals at the expected molecular weight of 155 kDa.
• The immunogen corresponds to the N-terminal region (amino acids 1–161), which may detect both full-length and alternative splice variants; validate specificity in the relevant sample system.
• In ELISA applications, the N-terminal targeting could be useful for capturing diverse MAP3K5 forms; employ appropriate assay optimization and controls.
• Given the cross-reactivity with human, mouse, and rat, this antibody is suitable for comparative studies across species, but always confirm reactivity in your specific model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials, including this high-quality anti-MAP3K5 polyclonal antibody for WB and ELISA applications. Our supply capabilities extend to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, all tailored to accelerate your apoptosis and cell signaling research. Partner with CamelBio for reliable, end-to-end procurement support from concept to clinical application.