Anti-MAP2K4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P45985

Core Product Specifications and Parameters

Biological Background: MAP2K4 Function and Localization

• Dual specificity protein kinase that acts as an essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway.
• Together with MAP2K7/MKK7, is one of the only known kinases to directly activate the stress-activated protein kinases JNK1, JNK2, and JNK3 by phosphorylation.
• Shows preference for phosphorylation of the tyrosine residue in the Thr-Pro-Tyr motif of JNKs, while MAP2K7 preferentially phosphorylates the threonine residue.
• In addition to JNKs, activates the p38 MAPKs MAPK11, MAPK12, MAPK13, and MAPK14, broadening its role in cellular stress responses.
• Required for maintaining peripheral lymphoid homeostasis; the MKK/JNK signaling pathway is involved in mitochondrial death signaling, including cytochrome c release and apoptosis.
• Localizes to both the cytoplasm and nucleus.
• Widely expressed with highest abundance in skeletal muscle.
• Post-translational modifications include acetylation, phosphorylation, and methylation, which regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300-399 of human MAP2K4; this region may confer cross-reactivity with mouse and rat orthologs, as indicated.
• For Western blotting, a band of approximately 44 kDa is expected. Validate specificity using knockout (KO)-validated lysates or appropriate positive/negative controls.
• For immunohistochemistry (paraffin), optimization of antigen retrieval and primary antibody dilution is recommended, considering tissue-specific expression patterns.
• Although validated for human, mouse, and rat, cross-reactivity with other species should be tested empirically if desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and researchers with quick access to validated antibodies such as anti-MAP2K4 polyclonal antibodies. We supply optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to streamline your assay development in the stress and immune signaling areas. Connect with us to accelerate your project from concept to clinic.