Anti-MAP2 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P11137

Core Product Specifications and Parameters

Biological Background: MAP2 Function and Localization

• MAP2 (Microtubule-associated protein 2) is a member of the MAP family, with multiple isoforms including MAP2A, MAP2B, and MAP2C, arising from alternative splicing.
• MAP2 binds to microtubules and is believed to stabilize them against depolymerization, contributing to cytoskeletal integrity.
• It also appears to exert a stiffening effect on microtubules, potentially influencing cellular mechanics.
• Subcellularly, MAP2 localizes to the cytoplasm, cytoskeleton, and specifically to dendrites in neurons, where it plays a critical role in dendritic structure.
• The protein contains calmodulin-binding domains and undergoes phosphorylation, suggesting regulation by calcium signaling and kinase pathways.
• MAP2 is classified as a phosphoprotein with repeat regions, and it serves as a reference proteome component, reflecting its fundamental importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-118) of human MAP2, which may detect multiple isoforms (MAP2A, MAP2B, MAP2C) depending on sequence conservation; verify isoform specificity in your experimental system.
• For Western blot (WB), the detected band is expected at approximately 200 kDa (full-length MAP2); endogenous MAP2 in neuronal lysates should run near this size; consider using mouse or rat brain extracts as positive controls.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), MAP2 signal should localize to dendrites and neuronal cell bodies; optimize fixation and permeabilization conditions for your sample type.
• For ELISA applications, the recombinant protein immunogen can serve as a positive control; note that polyclonal antibodies may exhibit lot-to-lot variation; include appropriate blocking and validation steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to premium IVD raw materials, including validated antibodies like this anti-MAP2 polyclonal antibody for neuroscience and cytoskeleton studies. Our portfolio covers optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your assay development from concept to clinic. Partner with us for reliable, one-stop procurement tailored to your IVD needs.