Anti-MAP2 Polyclonal Antibody for WB, IF, IHC, ELISA - P11137

Core Product Specifications and Parameters

Biological Background: MAP2 Function and Localization

• MAP2 (Microtubule-associated protein 2) is a neuronal protein that exists in multiple alternatively spliced isoforms, including MAP2A, MAP2B, MAP2C, and MAP2, with a full-length molecular weight of approximately 200 kDa (UniProt: P11137).
• MAP2 binds to microtubules and is thought to stabilize them against depolymerization, contributing to a stiffening effect on the cytoskeletal network.
• The protein localizes predominantly to the cytoplasm and cytoskeleton, with prominent enrichment in dendrites of neurons, playing a critical role in maintaining dendritic structure.
• MAP2 is subject to phosphorylation, which may regulate its microtubule-binding activity, and it contains calmodulin-binding domains, suggesting calcium-mediated signaling involvement.
• The protein features repetitive sequences that likely mediate its interaction with microtubules, and it is classified as a reference proteome component, indicating its well-characterized nature.
• MAP2 has been identified in multiple proteomics studies, confirming its widespread expression in neuronal tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 1510-1650) present in the longer isoforms of MAP2 (MAP2A and MAP2B); thus, the antibody may preferentially detect these high-molecular-weight forms in western blotting.
• For IF-P and IHC-P, consider using appropriate fixation and permeabilization methods to preserve microtubule structures and validate the antibody in the relevant sample system.
• Cross-reactivity with mouse and rat MAP2 has been reported; however, performance should be verified in the specific model organism.
• For western blotting, the expected band size is around 200 kDa, but smaller isoforms may also be detected depending on tissue type and alternative splicing.
• The antibody is provided as a polyclonal preparation, which may exhibit lot-to-lot variation; we recommend titrating and validating each new lot for your intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-MAP2 polyclonal antibody supports neuroscience and cytoskeleton research applications. CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development needs.