Anti-MAP1LC3C Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BXW4

Core Product Specifications and Parameters

Biological Background: MAP1LC3C Function and Localization

• MAP1LC3C is a member of the ATG8 family of ubiquitin-like modifiers, also known as autophagy-related protein LC3 C, MAP1 light chain 3-like protein 3, and MAP1A/MAP1B light chain 3C.
• Functions as a crucial ubiquitin-like modifier in antibacterial autophagy (xenophagy) by selectively binding CALCOCO2, recruiting all ATG8 family members to intracellular bacteria such as Salmonella typhimurium. Related references: PMID:23022382
• May participate in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins. Related references: PMID:28404643
• Localizes to cytoplasmic vesicles, autophagosome membranes, the endomembrane system, cytoplasm, and cytoskeleton, consistent with its autophagy-related roles.
• Most abundant in placenta, lung, and ovary, suggesting specialized tissue functions.
• Annotated as a phosphoprotein and processable through the ubiquitin-like conjugation pathway, indicating regulation by post-translational modifications for membrane association.
• Keywords: 3D-structure, Autophagy, Cytoplasmic vesicle, Cytoskeleton, Lipoprotein, Membrane, Microtubule, Phosphoprotein, Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen encompasses full-length human MAP1LC3C (residues 1-147); the antibody may recognize both unprocessed and lipidated forms, so consider validation in relevant autophagy models.
• For Western blotting, a band near 17 kDa is expected, but lipidation associated with autophagosome membranes can cause a mobility shift; consider evaluating in induced vs. basal autophagy conditions.
• In immunohistochemistry (IHC-P), optimize antigen retrieval protocols as the target resides on vesicular and membrane structures; appropriate controls are recommended.
• Cross-reactivity with mouse and rat predicted from sequence homology suggests utility in comparative preclinical studies, but empirical validation in each species is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the supply chain for IVD manufacturers and research teams with a portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials. This anti-MAP1LC3C antibody supports autophagy-focused assay development, and our team can help source complementary reagents—from recombinant MAP1LC3C proteins to matched detection pairs—to accelerate your project from concept to clinic.