Anti-MAP1LC3A Rabbit Monoclonal Antibody for WB, IP, ELISA - Q9H492

Core Product Specifications and Parameters

Biological Background: MAP1LC3A Function and Localization

• Microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), also known as LC3, ATG8E, or MAP1ALC3, is a core autophagy-related ubiquitin-like modifier essential for autophagosome formation.
• Plays a key role in the elongation of the phagophore membrane during macroautophagy, distinguishing its function from the GABARAP/GATE-16 subfamily, which acts primarily in autophagosome maturation. Related references: PMID:20713600
• Through interaction with the reticulophagy receptor TEX264, MAP1LC3A participates in remodeling endoplasmic reticulum subdomains into autophagosomes under nutrient stress, facilitating ER turnover via selective autophagy. Related references: PMID:31006537 PMID:31006538
• Localizes to cytoplasmic autophagosome membranes and is also associated with the cytoskeleton and endomembrane system, reflecting its dynamic role in vesicle trafficking and autophagy.
• Tissue expression is most abundant in heart, brain, liver, skeletal muscle, and testis, with absence in thymus and peripheral blood leukocytes, suggesting tissue-specific autophagic activity.
• Classified under keywords including autophagy, Ubl conjugation pathway, and alternative splicing, highlighting its processing and conjugation mechanisms critical for function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to N-terminal residues 1–100; the antibody may recognize both full-length and processed (lipidated) forms of LC3A. Validate detection specificity in your experimental system.
• For Western blot, expect a band near 14–16 kDa; the lipidated LC3-II form migrates faster than the cytosolic LC3-I form. Use lysosomal inhibitors (e.g., chloroquine or bafilomycin A1) to monitor autophagic flux.
• This antibody is suitable for immunoprecipitation and ELISA. Optimize antibody concentrations empirically, and include appropriate controls for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with high-quality IVD raw materials, technical services, and consulting support from concept to clinical application. For autophagy-related targets like MAP1LC3A, our sourcing capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target procurement to streamline your development workflow. Let CamelBio be your partner in advancing autophagy research and clinical diagnostics.