Anti-MAOB Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P27338

Core Product Specifications and Parameters

Biological Background: MAOB Function and Localization

• Monoamine oxidase B (MAOB) is a mitochondrial outer membrane enzyme that catalyzes the oxidative deamination of primary and some secondary amines, playing a key role in the metabolism of neurotransmitters and vasoactive amines.
  Related references: PMID:11049757 PMID:11134050 PMID:20493079
• It preferentially degrades benzylamine and phenylethylamine, and also processes the neurotoxin MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine).
  Related references: PMID:11049757 PMID:11134050 PMID:20493079
• MAOB requires FAD as a cofactor and is a member of the flavin-containing amine oxidase family; it is localized to the mitochondrial outer membrane with a single transmembrane helix.
• Post-translational modifications include acetylation, and alternative splicing produces multiple isoforms.
• The protein has a canonical molecular weight of approximately 58.8 kDa, but typically migrates at 59 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 400-490 in the C-terminal region of human MAOB. When designing epitope mapping or competition assays, consider this specific region.
• For Western blotting, anticipate a band around 59 kDa; validate detection in relevant cell or tissue lysates (e.g., brain, liver, kidney) using appropriate loading controls.
• In immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), optimize antigen retrieval and permeabilization conditions; strong mitochondrial outer membrane staining should be expected.
• This antibody shows cross-reactivity with mouse and rat MAOB, making it suitable for translational studies in rodent models; confirm specificity in your model system.
• For ELISA applications, pair this polyclonal antibody with appropriate MAOB standards and detection reagents, and validate the assay’s dynamic range and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibodies such as this anti-MAOB polyclonal antibody. Our portfolio encompasses targets across neuroscience and metabolism research, supported by bulk antibody production, customized reagent sourcing, and technical consultation to streamline your assay development from concept to clinical application.