Anti-MANBA Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O00462

Core Product Specifications and Parameters

Biological Background: MANBA Function and Localization

• Beta-mannosidase (MANBA), also known as lysosomal beta A mannosidase or mannanase, is encoded by the MANBA gene (GeneID: 4126). It belongs to the glycosyl hydrolase family and functions as an exoglycosidase.
• The enzyme specifically cleaves the single beta-1,4-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides, playing a critical role in glycoprotein catabolism.
• MANBA is localized within lysosomes, where it participates in the stepwise degradation of N-glycans.
• It is ubiquitously expressed, with higher levels detected in pancreas, kidney, and placenta, and lower levels in liver, lung, brain, heart, and muscle.
• The protein is a glycoprotein with disulfide bonds, and it contains a signal peptide for lysosomal targeting.
• Keywords associated with MANBA include: Disulfide bond, Glycoprotein, Glycosidase, Hydrolase, Lysosome, Proteomics identification, Reference proteome, Signal.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 18-300 of human MANBA, which encompasses the mature protein excluding the signal peptide, suggesting potential recognition of the native, processed form.
• For Western blot, a band around 101 kDa is expected; however, due to glycosylation, additional bands or a shift may occur. Consider using a reducing agent and loading appropriate controls.
• In immunohistochemistry (paraffin), antigen retrieval methods may be necessary; validate staining in known positive tissues (e.g., pancreas or kidney).
• ELISA can be used for quantitative detection; pair with a validated secondary antibody and optimize coating antigens.
• Cross-reactivity with mouse is reported; sequence alignment shows high homology, but validate in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For beta-mannosidase research and lysosomal storage disease studies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.