Anti-MAN2B1 Rabbit Polyclonal Antibody for WB, ELISA - O00754

Core Product Specifications and Parameters

Biological Background: MAN2B1 Function and Localization

• MAN2B1 encodes lysosomal alpha-mannosidase (also known as lysosomal acid alpha-mannosidase, mannosidase alpha class 2B member 1, or mannosidase alpha-B; gene aliases LAMAN, MANB).
• The enzyme hydrolyzes terminal alpha-mannosidic linkages on oligosaccharides generated during N-glycoprotein degradation, cleaving alpha 1,2-, 1,3-, and 1,6-linked mannose residues.
• It belongs to the glycoside hydrolase family 38 and is essential for the catabolism of asparagine-linked glycoproteins.
• Localized predominantly in the lysosome lumen, with a fraction also secreted into the extracellular space.
• Synthesized as a zymogen and processed via proteolytic cleavage; contains disulfide bonds and undergoes N-linked glycosylation.
• Requires a zinc ion for catalytic activity, as indicated by metal-binding properties.
• Mutations in MAN2B1 cause alpha-mannosidosis, a lysosomal storage disorder (keyword: Disease variant).
• Multiple isoforms are produced through alternative splicing (keyword: Alternative splicing).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 640-850) of human MAN2B1; consider its relevance for detecting specific isoforms or processed forms.
• For Western blot, use standard reducing conditions and a predicted molecular weight of approximately 114 kDa; validate detection in relevant cell or tissue lysates, including mouse samples due to cross-reactivity.
• In ELISA applications, optimize coating buffer, blocking agent, and antibody dilutions; recombinant MAN2B1 or peptide may serve as positive controls.
• Lysosomal pre-treatment or enrichment may enhance signal in samples with low endogenous expression.
• Cross-reactivity with mouse reported; verify specificity in knockout models or by peptide competition if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a consolidated supply chain for glycobiology and lysosomal disease research. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets—ensuring continuity and reliability for diagnostic manufacturers and laboratories. With this anti-MAN2B1 antibody, focus your efforts on advancing lysosomal storage disorder studies while CamelBio handles the procurement complexities.