Anti-MAN2A2 Polyclonal Antibody for WB, ELISA - P49641

Core Product Specifications and Parameters

Biological Background: MAN2A2 Function and Localization

• MAN2A2 encodes alpha-mannosidase 2x (Man IIx), also known as mannosidase alpha class 2A member 2, a key glycosyl hydrolase involved in N-glycan maturation.
• The enzyme catalyzes the first committed step in the biosynthesis of complex N-glycans by converting high-mannose intermediates to hybrid and complex structures through the removal of alpha-1,3- and alpha-1,6-mannose residues.
• MAN2A2 functions as the final hydrolytic enzyme in the N-glycan processing pathway within the Golgi apparatus, ensuring proper glycoprotein quality control and secretion.
• The protein is localized to the Golgi apparatus membrane via a signal-anchor transmembrane domain, placing its catalytic domain in the lumen for glycan trimming.
• As a member of the glycosyl hydrolase family 38, MAN2A2 requires zinc as a metal cofactor for its enzymatic activity and contains disulfide bonds critical for structural stability.
• Alternative splicing of MAN2A2 generates multiple isoforms, which may exhibit distinct substrate specificities or regulatory roles in glycoprotein biosynthesis.
• Keywords associated with this protein highlight its involvement in glycosylation, hydrolase activity, and membrane association, underscoring its role in post-translational modification networks.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from amino acids 100-200 of human MAN2A2; ensure this region is conserved in your target samples and isoforms.
• For Western blot, a band of approximately 131 kDa is expected; consider using a high molecular weight marker and validate with positive controls expressing MAN2A2 (e.g., HeLa or HEK293 cells).
• In ELISA applications, titrate the antibody and optimize coating conditions to assess specificity against recombinant MAN2A2 or cell lysates.
• Avoid repeated freeze-thaw cycles and aliquot upon receipt to maintain antibody stability, as recommended for polyclonal antibodies.
• Cross-reactivity with other species has not been tested; validate in the relevant sample system if using non-human models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting diagnostic developers and researchers with validated antibodies like this anti-MAN2A2 polyclonal antibody. Our portfolio includes bulk ancillary reagents, optimized antibody pairs, and rare target sourcing to streamline glycobiology and N-glycosylation assay development. Partner with us to bridge your concept-to-clinic needs efficiently.