Anti-MAN2A1 Polyclonal Antibody for WB, ELISA - Q16706

Core Product Specifications and Parameters

Biological Background: MAN2A1 Function and Localization

• MAN2A1 encodes alpha-mannosidase 2 (EC 3.2.1.114), also known as Golgi alpha-mannosidase II (AMan II), a key hydrolase involved in protein N-glycosylation.
• The enzyme controls the final hydrolytic step in the maturation pathway of N-glycans, catalyzing the conversion of high-mannose to complex N-glycans.
• This reaction is the first committed step in the biosynthesis of complex N-glycans, essential for proper glycoprotein processing and function.
• MAN2A1 is localized to the Golgi apparatus membrane, consistent with its role in post-translational modification within the secretory pathway.
• The protein contains a signal-anchor and a transmembrane helix, tethering it to the membrane; it belongs to the glycoside hydrolase family 38.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and phosphorylation, as identified in proteomic studies.
• MAN2A1 requires zinc for activity and is classified as a metal-binding enzyme, highlighting its catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 865–1144 in the C-terminal region of human MAN2A1; antibody reactivity may include full-length and processed forms.
• For Western blotting, consider using reducing denaturing conditions and a protein ladder covering the predicted band at approximately 131 kDa.
• Cross-reactivity with mouse and rat samples indicates conserved epitopes; validate detection in your specific experimental model.
• In ELISA applications, optimize coating concentration and detection system to account for polyclonal binding.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio provides access to MAN2A1 antibodies and ancillary reagents for N-glycosylation research, from discovery through diagnostic development. Our one-stop sourcing model supports your assay innovation with validated polyclonal antibodies, bulk formats, and tailored technical consultation. Explore our portfolio for glycosylation-related targets and rely on our supply chain expertise to secure rare or specialized raw materials efficiently.