Anti-MAN1C1 Rabbit Polyclonal Antibody for WB and ELISA - Q9NR34

Core Product Specifications and Parameters

Biological Background: MAN1C1 Function and Localization

• MAN1C1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase IC) is a Golgi-resident alpha-1,2-mannosidase that processes N-linked glycans by sequentially trimming terminal mannose residues from high-mannose precursors, converting Man9GlcNAc2 to Man8, Man6, and minor Man5 species.
• It is a member of the glycosyl hydrolase family 47, also known as class I alpha-mannosidases, which are calcium-dependent enzymes critical for glycoprotein quality control and trafficking.
• The protein localizes to the Golgi apparatus membrane via a signal-anchor transmembrane domain, consistent with its role in the early secretory pathway.
• MAN1C1 is broadly expressed across human tissues, with notably high expression in placenta; it is absent or low in lung, muscle, and pancreas.
• Post-translational modifications include N-glycosylation and phosphorylation at multiple sites, and the enzyme's activity requires binding of calcium and other divalent metal ions.
• Structural features include disulfide bonds that stabilize the catalytic domain, typical of secreted and membrane-associated glycosidases.
• Also known by aliases HMIC, MAN1C, MAN1A3, and pp6318, this protein is a validated human target identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (aa44–240) within the catalytic domain, supporting cross-reactivity with human and mouse MAN1C1; orthologue alignment verification is recommended before use in other species.
• For western blotting, the predicted molecular weight is approximately 71 kDa; consider that glycosylation may cause a slightly higher apparent mass.
• For ELISA, consider pairing with a monoclonal antibody specific to a non-overlapping epitope or using appropriate blocking to minimize non-specific binding.
• Optimize detergent and salt concentrations in lysis buffers to solubilize this membrane protein effectively.
• Validate the antibody in the relevant biological context (e.g., Golgi-enriched fractions) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies for glycan-processing enzymes like MAN1C1. Our one-stop sourcing platform offers optimized polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to accelerate assay development. Whether you need rare-target antibodies or large-scale production, CamelBio bridges the gap from concept to clinic.