Anti-MAML2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IZL2

Core Product Specifications and Parameters

Biological Background: MAML2 Function and Localization

• MAML2 (Mastermind-like protein 2) acts as a transcriptional coactivator for NOTCH proteins, amplifying NOTCH-induced transcription of targets such as HES1.
• It preferentially potentiates activation by NOTCH3 and NOTCH4 compared to MAML1 or MAML3.
• The protein localizes to nuclear speckles, consistent with its role in transcriptional regulation.
• MAML2 is widely expressed across human tissues, with highest levels detected in placenta, salivary gland, and skeletal muscle.
• As a key component of the Notch signaling pathway, MAML2 plays a role in cell fate determination, development, and oncogenesis.
• The protein is subject to phosphorylation, which may regulate its coactivator activity or stability.
• Genetic rearrangements involving the MAML2 gene are linked to certain mucoepidermoid carcinomas.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 750-850 of human MAML2, a region conserved across human, mouse, and rat, supporting predicted cross-reactivity.
• For Western blot, the predicted molecular weight is ~125 kDa; consider using appropriate gel percentage and transfer conditions.
• In immunofluorescence/ICC, nuclear speckle localization may be observed; co-staining with Notch pathway markers could be informative.
• ELISA can be used for antibody titer determination or antigen detection; include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents, supporting your Notch signaling research and diagnostic development. Our one-stop sourcing platform streamlines procurement from concept to clinic, offering rare target antibodies like anti-MAML2 and tailored technical consulting.