Anti-MAML1 Rabbit Polyclonal Antibody for WB, ELISA - Q92585

Core Product Specifications and Parameters

Biological Background: MAML1 Function and Localization

• MAML1 (Mastermind-like protein 1) is a transcriptional coactivator essential for Notch signaling, amplifying Notch-induced transcription of target genes such as HES1.
• It binds to the Notch intracellular domain (NICD) and recruits coactivators like CREBBP/CBP, promoting nucleosome acetylation at Notch enhancers to activate transcription.
• MAML1 enhances phosphorylation and proteolytic turnover of NICD through interaction with CDK8, providing a mechanism for signal termination.
• The protein localizes to nuclear speckles, subnuclear structures enriched in pre-mRNA splicing factors, implying a role in coupling transcription to RNA processing.
• MAML1 is widely expressed, with highest levels in heart, pancreas, peripheral blood leukocytes, and spleen, consistent with its role in hematopoietic development.
• It regulates Notch-mediated lymphoid cell fate decisions, influencing T versus B cell lineage commitment in the hematopoietic system.
• Post-translational modifications include phosphorylation and acetylation, which may modulate its activity and protein interactions.
• Keywords associated with MAML1: 3D-structure, acetylation, activator, Notch signaling pathway, nucleus, phosphoprotein.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of MAML1 is ~108 kDa; however, due to post-translational modifications, the observed band may vary. Consider using nuclear extracts from appropriate cell lines (e.g., HeLa, Jurkat) and validate in the relevant sample system.
• As a polyclonal antibody, it may exhibit reactivity against other mastermind-like family members (MAML2, MAML3); sequence alignment of the immunogen region is recommended to assess potential cross-reactivity.
• For ELISA, titrate the antibody to optimize the signal-to-noise ratio using recombinant MAML1 protein or peptide as coating antigen, and validate in your specific assay conditions.
• The immunogen spans amino acids 1-300 of human MAML1, a region that may be conserved across species; the reported cross-reactivity with mouse and rat should be confirmed in your model system.
• Store the antibody as per standard protocols for polyclonal antibodies, and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to IVD raw materials, technical services, and consulting. Our MAML1 rabbit polyclonal antibody supports research into Notch signaling and transcriptional regulation, and we can supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development from concept to clinic.