Anti-MAK16 Rabbit Polyclonal Antibody for WB, ELISA - Q9BXY0

Core Product Specifications and Parameters

Biological Background: MAK16 Homolog Function and Localization

• Protein MAK16 homolog, also known as RBM13 or NNP78, is encoded by the MAK16 gene (GeneID: 84549).
• It localizes to the nucleus, specifically the nucleolus, as indicated by UniProt annotation (Q9BXY0).
• The protein is subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination (Ubl conjugation), with evidence of isopeptide bond formation.
• Its molecular weight is approximately 35 kDa (calculated from sequence: 35,369 Da).
• Structural information is available, as noted by the 3D-structure keyword in UniProt, although specific PDB entries are not provided in this context.
• Alternative names RBM13 suggest an RNA-binding motif, consistent with its nucleolar localization and potential involvement in ribosomal RNA processing.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 30-120 of human MAK16, an N-terminal region that may be conserved across species; validate cross-reactivity in mouse and rat samples as indicated.
• For Western blot (WB), a band around 35 kDa is expected; consider using whole-cell lysates from human, mouse, or rat cell lines, and optimize antibody dilution empirically.
• For ELISA, the antibody can be used as a capture or detection reagent; pair with a compatible secondary antibody and validate with recombinant MAK16 protein or peptide.
• Because MAK16 is a nucleolar protein, nuclear enrichment protocols may enhance signal in WB or other assays.
• Standard storage at -20°C or below with ice bag shipping is recommended to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to IVD raw materials, technical services, and consultative support from concept to clinic. The MAK16 rabbit polyclonal antibody, targeting a nucleolar protein implicated in ribosome biogenesis, exemplifies our commitment to supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies, and rare target reagents. As a one-stop sourcing partner, we help accelerate your assay development and ensure reliable material supply for your diagnostic needs.