Anti-MAK Polyclonal Antibody for WB, IHC-P, ELISA - P20794

Core Product Specifications and Parameters

Biological Background: MAK Function and Localization

• Serine/threonine-protein kinase MAK (male germ cell-associated kinase) is encoded by the MAK gene and expressed as two major isoforms via alternative splicing; isoform 1 is widely expressed, while isoform 2 is retina-specific.
• MAK is essential for the regulation of ciliary length and is required for the long-term survival of photoreceptors, highlighting its critical role in ciliary biology and retinal health.
• The kinase phosphorylates FZR1 in a cell cycle-dependent manner and functions as a transcriptional coactivator of the androgen receptor (AR), linking it to cell cycle control and hormone signaling.
• MAK is implicated in spermatogenesis, consistent with its original identification as a male germ cell-associated kinase.
• Subcellularly, MAK localizes to the nucleus, cytoplasm, centrosome, spindle, midbody, and photoreceptor inner and outer segments, reflecting its dynamic roles in cell division and ciliary function.
• Tissue expression is elevated in prostate cancer cell lines compared to normal prostate epithelia; isoform 1 is found in kidney, testis, lung, trachea, and retina, while isoform 2 is restricted to rod and cone photoreceptors.
• Key structural features include an ATP-binding site and kinase domain, and MAK belongs to a group of proteins associated with retinitis pigmentosa when mutated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 370-435) of human MAK, which may contribute to isoform-specific recognition; consider verifying isoform detection in your system.
• For Western blot, the predicted protein weight is approximately 71 kDa; ensure adequate gel resolution and transfer conditions.
• In IHC-P, signal may be observed in tissues with known expression (e.g., retina, testis, prostate cancer specimens); appropriate antigen retrieval and antibody titration are recommended.
• Cross-reactivity with mouse and rat is reported, enabling translational studies; validate the antibody in the relevant species and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering reliable access to validated antibodies, including this anti-MAK polyclonal antibody, which supports research in ciliary biology, cancer, and reproductive science. Our one-stop platform streamlines procurement of monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target materials, empowering diagnostic manufacturers and labs from concept to clinic. Let CamelBio be your trusted bridge to high-quality, cost-effective raw material supply for your next project.