Anti-Major Vault Protein (MVP) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14764

Core Product Specifications and Parameters

Biological Background: Major Vault Protein (MVP) Function and Localization

• MVP (Major vault protein), also known as lung resistance-related protein (LRP) or VAULT1, is the main structural component of vault particles—large ribonucleoprotein complexes implicated in cargo transport and signal transduction. Related references: PMID:40691181
• The protein oligomerizes to form a cage-like vault structure that can encapsulate minor vault components such as PARP4. Related references: PMID:40691181
• Vaults are believed to participate in nucleo-cytoplasmic transport, shuttling molecules between the nucleus and cytoplasm. Related references: PMID:15133037
• MVP negatively regulates interferon-gamma (IFNG)-mediated STAT1 signaling and downstream JAK activation. Related references: PMID:16418217
• MVP down-regulates SRC activity and MAP kinase signaling pathways. Related references: PMID:16441665
• Subcellularly, MVP localizes to the cytoplasm, nuclear pore complexes, and perinuclear region, consistent with its transport roles.
• MVP is expressed in most normal tissues, with higher levels in epithelial cells with secretory/excretory functions and in cells chronically exposed to xenobiotics; it is frequently overexpressed in multidrug-resistant cancer cells.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, and the protein features multiple repeat domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–260) of human MVP; consider this when interpreting band patterns or cross-reactivity in orthologs.
• For Western blot (WB), the predicted molecular weight is ~99 kDa; however, note that MVP can form high-molecular-weight complexes, so verify under reducing conditions.
• For immunohistochemistry (IHC-P), optimal dilution and antigen retrieval should be determined empirically for each tissue type.
• In ELISA, the antibody may be used as capture or detection reagent; validate pairing with appropriate secondary antibodies.

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