Anti-Major Vault Protein (LRP/MVP) Monoclonal Antibody for WB, IHC-P, ELISA - Q14764

Core Product Specifications and Parameters

Biological Background: Major Vault Protein Function and Localization

• Major vault protein (MVP), also known as lung resistance-related protein (LRP), is the main structural component of the vault particle, a large ribonucleoprotein complex.
• It oligomerizes to form a cage-like vault complex that can encapsulate minor components like PARP4, suggesting a role in cargo transport and signal transduction. Related references: PMID:40691181
• MVP is implicated in nucleo-cytoplasmic transport, potentially shuttling cargo between the cytoplasm and the nucleus through the nuclear pore complex. Related references: PMID:15133037
• The protein negatively regulates IFNG-mediated STAT1 signaling and subsequent JAK activation, thereby modulating immune responses. Related references: PMID:16418217
• MVP down-regulates SRC activity and MAP kinase signaling, indicating a role in controlling cell proliferation and survival pathways. Related references: PMID:16441665
• Subcellularly, MVP localizes to the cytoplasm, perinuclear region, nucleus, and nuclear pore complexes, consistent with its transport function.
• It is present in most normal tissues, with higher expression in epithelial cells with secretory and excretory functions, and is often overexpressed in multidrug-resistant cancer cells.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond), and it is identified as a phosphoprotein and ribonucleoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-370) of human MVP, which is likely to generate antibodies recognizing the native protein; consider epitope accessibility in different applications.
• For Western blotting, the predicted molecular weight of MVP is approximately 99 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P), antigen retrieval methods may be required; validate in tissues with known MVP expression, such as bronchial epithelium or intestine.
• Cross-reactivity with mouse and rat allows for comparative studies; confirm consistency in model systems.
• For ELISA, optimize coating antigen and antibody dilution to achieve linear detection range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing solutions. This anti-MVP antibody supports research in multidrug resistance, cancer biology, and nucleocytoplasmic transport, and we offer comprehensive materials to streamline your assay development. Contact us to leverage our full procurement capabilities from concept to clinic.