Anti-MAGP-2/MFAP5 Rabbit Monoclonal Antibody for WB, ELISA - Q13361

Core Product Specifications and Parameters

Biological Background: MAGP-2/MFAP5 Function and Localization

• MAGP-2/MFAP5 (Microfibrillar-associated protein 5), also known as MP25 or MAGP-2, is a 20 kDa secreted extracellular matrix protein encoded by the MFAP5 gene (GeneID: 8076).
• It is a component of elastin-associated microfibrils, contributing to the structural integrity of connective tissues. Related references: PMID:8557636
• It may play a role in hematopoiesis and, in the cardiovascular system, could regulate growth factors or participate in cell signaling to maintain large vessel integrity (By similarity).
• The protein is secreted and localizes to the extracellular space and extracellular matrix, consistent with its role in microfibril assembly.
• Post-translational modifications include glycosylation and the formation of disulfide bonds, as indicated by UniProt keywords.
• Alternative splicing and disease-associated variants have been reported, with mutations linked to aortic aneurysm.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using denaturing and reducing conditions, as disulfide bonds may affect migration; a band around 20 kDa is expected.
• For ELISA, validate the antibody with recombinant or native MAGP-2/MFAP5 under appropriate coating and blocking conditions.
• The antibody is reported to react with human samples; if cross-reactivity with other species is required, independent validation is recommended.
• Optimize antibody dilution and incubation times based on your specific experimental setup and detection systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined one-stop channel for IVD raw materials, technical support, and consulting—from concept to clinic. This MAGP-2/MFAP5 antibody supports research in extracellular matrix biology, connective tissue disorders, and cardiovascular disease, with applications in WB and ELISA. Our comprehensive supply portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials.