Anti-MAGED1/NRAGE Rabbit Monoclonal Antibody for WB, ELISA - Q9Y5V3

Core Product Specifications and Parameters

Biological Background: MAGED1/NRAGE Function and Localization

• MAGED1 (Melanoma-associated antigen D1), also known as NRAGE or DLXIN-1, is a member of the MAGE protein family and functions in apoptosis, cell cycle regulation, and circadian rhythm modulation.
• The protein is involved in NGF-induced neuronal apoptosis by inhibiting cell cycle progression and facilitating NGFR-mediated apoptosis.
• MAGED1 may act as a regulator of DLX family transcription factors and enhance the ubiquitin ligase activity of RING-type E3 ligases, potentially through recruitment/stabilization of the E2 enzyme.
• It plays a role in circadian rhythm by acting as a co-regulator of RORA, modulating clock genes such as BMAL1, NFIL3, and NR1D1.
• Subcellularly, MAGED1 localizes to the cytoplasm, cell membrane, and nucleus, consistent with its multifunctional roles.
• The protein is ubiquitously expressed, with documented expression in bone marrow stromal cells from both healthy donors and multiple myeloma patients.
• Post-translational modifications include phosphorylation, and alternative splicing produces multiple isoforms.
• Keywords associated with MAGED1 include tumor antigen, Ubl conjugation pathway, biological rhythms, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 640-778) of human MAGED1, suggesting that the antibody may recognize epitopes within this domain; consider validating specificity in knockout or knockdown models if available.
• For Western blot (WB), the predicted molecular weight is approximately 86 kDa; however, potential splice variants or post-translational modifications could result in additional bands; optimize blocking and detection conditions accordingly.
• This rabbit monoclonal antibody is suitable for ELISA; consider testing a range of concentrations for coating and detection to establish optimal signal-to-noise ratio.
• Cross-reactivity is indicated for human; if working with other species, verify sequence homology within the immunogen region to predict potential reactivity.
• As MAGED1 localizes to multiple subcellular compartments (cytoplasm, membrane, nucleus), appropriate sample preparation (e.g., subcellular fractionation) may enhance detection in specific compartments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-MAGED1/NRAGE product, essential for apoptosis, circadian rhythm, and cancer research. Our supply portfolio covers bulk ancillary reagents and sourcing for rare targets, supporting your development from concept to clinic. Contact us for customized procurement solutions tailored to your production needs.