Anti-MAGEA6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P43360

Core Product Specifications and Parameters

Biological Background: MAGEA6 Function and Localization

• MAGEA6 (Melanoma-associated antigen 6), also designated Cancer/testis antigen 1.6 (CT1.6) or MAGE3B, is a member of the MAGE family of tumor antigens.
• Functions as an activator of ubiquitin ligase activity of RING-type E3 ubiquitin-protein ligases and acts as a repressor of autophagy. Related references: PMID:17942928 PMID:20864041 PMID:31267705
• Enhances ubiquitin ligase activity of TRIM28, thereby stimulating p53/TP53 ubiquitination; proposed to act through recruitment or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Related references: PMID:17942928 PMID:20864041
• May play a role in tumor transformation or aspects of tumor progression; in vitro, it promotes cell viability in melanoma cell lines. Related references: PMID:17942928
• Expressed in a variety of tumors including melanoma, head and neck squamous cell carcinoma, lung carcinoma, and breast carcinoma, but not in normal tissues except for testes.
• Classified under tumor antigens and the ubiquitin conjugation pathway, reflecting its role in protein modification and cancer biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 75–314 of human MAGEA6; this region may be conserved across species, supporting detection in human and mouse (validated cross-reactivity).
• For Western blot (WB), consider using whole cell lysates from testis tissue or MAGEA6-positive tumor cell lines; the predicted molecular weight is ~35 kDa, though post-translational modifications may alter migration.
• For immunofluorescence/ICC, validate in cell lines known to express MAGEA6; appropriate fixation and permeabilization should be optimized.
• The polyclonal nature may provide robust signal but could result in detection of non-specific bands; include appropriate controls.
• ELISA applications can be developed using this antibody as a capture or detection reagent; pairing with a monoclonal detection antibody may improve specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, connecting diagnostic developers with high-quality antibodies like this MAGEA6 polyclonal antibody, which targets a critical cancer/testis antigen for oncology research and assay development. Our portfolio includes validated antibody pairs, bulk polyclonal and monoclonal antibodies, ancillary reagents, and custom sourcing for rare targets—streamlining your path from concept to clinic. Let CamelBio support your next-generation diagnostic kits with reliable, scalable raw material solutions.