Anti-MAGEA4 Rabbit Polyclonal Antibody for WB, ELISA - P43358

Core Product Specifications and Parameters

Biological Background: MAGEA4 Function and Localization

• MAGEA4 (Melanoma-associated antigen 4) is a protein encoded by the MAGEA4 gene and belongs to the MAGE family of cancer/testis antigens. It is also known as CT1.4, MAGE-41, and MAGE-X2.
• The protein plays a role in cell cycle regulation; it inhibits cell cycle arrest at the G1 phase, thereby promoting cell proliferation. Additionally, it negatively regulates p53-mediated apoptosis. Related references: PMID:22842486
• MAGEA4 expression is highly restricted; it is found in many tumor types (melanoma, head and neck squamous cell carcinoma, lung carcinoma, breast carcinoma) but is absent from normal tissues except the testes and placenta, making it a classic cancer/testis antigen.
• Biochemically, the protein has a predicted molecular weight of approximately 35 kDa and is identified in proteomics datasets.
• According to UniProt, MAGEA4 features a known 3D structure and is classified under the keywords "Tumor antigen" and "Reference proteome".

Experimental Guidance and Technical Tips

• The immunogen spans a large portion of MAGEA4 (aa 28-317), which may result in a polyclonal antibody with multiple epitopes; consider this for applications like sandwich ELISA where a pair with another clone might be needed.
• For Western blot, a band around 35 kDa is expected; optimize protein loading and detection conditions according to the sample's expression level (high in tumors, low in normal tissues).
• Given cross-reactivity with human, mouse, and rat, the antibody likely targets a conserved region; however, always validate in the relevant species and sample type.
• For ELISA applications, consider using the antibody as a capture or detection reagent, noting that polyclonal antibodies may provide broad reactivity.
• Since MAGEA4 is a tumor antigen, it is advisable to use tumor cell lines or tissue lysates as positive controls, and normal tissue (excluding testis/placenta) as negative controls in immunodetection assays.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop supplier, CamelBio provides this anti-MAGEA4 rabbit polyclonal antibody alongside a broad portfolio of IVD raw materials, including validated antibody pairs and bulk reagents. We cater to diagnostic manufacturers and research institutes, facilitating seamless procurement of rare and standard targets like cancer/testis antigens.