Anti-MAGE-1/MAGE1A Rabbit Polyclonal Antibody for WB - P43355

Core Product Specifications and Parameters

Biological Background: MAGE-1/MAGE1A Function and Localization

• Known as Cancer/testis antigen 1.1 (CT1.1) and MAGE-1 antigen, the MAGEA1 protein belongs to the melanoma-associated antigen family.
• May participate in transcriptional regulation by interacting with SNW1 and recruiting histone deacetylase HDAC1.
• Possibly inhibits Notch intracellular domain (NICD) transactivation, contributing to developmental and oncogenic processes.
• Plays a potential role in embryonal development and tumor transformation or aspects of tumor progression.
• Recognized as a tumor antigen by autologous cytolytic T-lymphocytes in melanoma.
• Found in both cytoplasm and nucleus.
• Tissue specificity: expressed in many tumors such as melanoma, head and neck squamous cell carcinoma, lung carcinoma, and breast carcinoma, but not in normal tissues except testis; absent in kidney tumors, leukemias, and lymphomas.
• Annotated as a phosphoprotein and a tumor antigen involved in transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full length of MAGE-1/MAGE1A (amino acids 1–309), making this polyclonal antibody likely to recognize multiple epitopes and providing robust detection across assays.
• For Western blot, expect a band around 34 kDa; validate using MAGE-1 positive cell lines (e.g., melanoma-derived).
• In IHC-P and IF/ICC, optimize antigen retrieval and include appropriate positive and negative controls to assess specificity.
• For ELISA, the polyclonal nature can offer enhanced sensitivity; consider coating with immunogen or recombinant protein as capture.
• Cross-reactivity with other MAGE family members has not been characterized; perform sequence alignment if multi-family recognition is a concern.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This MAGE-1/MAGE1A antibody supports oncology research and diagnostic development targeting cancer/testis antigens. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials to streamline your assay development.