Anti-MAG (Myelin-Associated Glycoprotein) Polyclonal Antibody for WB and ELISA - P20916

Core Product Specifications and Parameters

Biological Background: MAG Function and Localization

• Myelin-associated glycoprotein (MAG), also designated Siglec-4a, is encoded by the MAG gene (GeneID: 4099) and exists as two major isoforms (isoform 1 and isoform 2) produced by alternative splicing.
• MAG functions as an adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.
• It is not essential for initial myelination but is critical for the maintenance of normal axon myelination and for preventing degeneration of myelinated axons in adults.
• MAG acts as a negative regulator of neurite outgrowth, inhibiting axonal extension through binding to RTN4R/RTN4RL2 or gangliosides, depending on the neuronal cell type.
• Subcellularly, MAG is localized to the cell membrane and membrane rafts of myelinating glial cells.
• Both isoforms are abundantly expressed in myelinated structures of the central and peripheral nervous systems, including oligodendroglia, periaxonal myelin, and Schmidt-Lanterman incisures.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 20-220) of human MAG, which is likely to generate antibodies recognizing both isoforms of the endogenous protein.
• For Western blot, the predicted molecular weight of MAG is approximately 69 kDa; use appropriate gel percentage and transfer conditions for this size.
• Validate antibody specificity in relevant sample systems such as brain or spinal cord tissue lysates, where MAG is highly expressed.
• Cross-reactivity with mouse and rat MAG is reported, making this antibody suitable for studies in rodent models.
• For ELISA applications, consider using the recombinant immunogen as a positive control to establish assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to premium IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. This anti-MAG polyclonal antibody is an ideal raw material for neuroscience and neurology IVD assay development, benefiting from CamelBio’s validated antibody pair sourcing, monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement capabilities. Partner with us to streamline your next-generation diagnostic product pipelines.