Anti-MAG Monoclonal Antibody for WB, IHC-P, IF-F, IP, ELISA - P20916

Core Product Specifications and Parameters

Biological Background: MAG Function and Localization

• MAG (Myelin-associated glycoprotein), also known as Siglec-4a, is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• It mediates cell-cell adhesion between myelinating cells and neurons by binding to sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.
• Plays a crucial role in maintaining axon myelination and protecting motoneurons from apoptosis, particularly after injury.
• Acts as a negative regulator of neurite outgrowth; in sensory neurons, this inhibition depends only partially on RTN4R, RTN4RL2, and gangliosides.
• Preferentially binds to alpha-2,3-linked sialic acid and specific gangliosides such as Gt1b.
• Localizes to the cell membrane and membrane rafts; in the nervous system, it is detected in periaxonal myelin and Schmidt-Lanterman incisures, with two isoforms (isoform 1 and isoform 2) expressed in both central and peripheral nervous system myelin (PubMed:6200494, PubMed:9495552). Related references: PMID:6200494 PMID:9495552

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band around 69 kDa; consider using reducing conditions and whole cell lysates from neuronal or glial cell lines as positive controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-F), antigen retrieval may be necessary; validate with human, mouse, or rat brain tissue sections.
• For immunoprecipitation (IP) and ELISA, test antibody performance with recombinant MAG protein or tissue lysates; cross-reactivity with mouse and rat suggests broad use in rodent models.
• In neurite outgrowth assays, be aware that MAG’s inhibitory effects are cell-type specific; co-culture models with neurons and myelinating cells may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-MAG antibody supports neurobiology research and diagnostic development targeting myelination and neurodegenerative diseases. With capabilities spanning from rare target sourcing to technical consulting, CamelBio accelerates your workflow from concept to clinic.