Anti-MAG Monoclonal Antibody for WB, ELISA - P20916

Core Product Specifications and Parameters

Biological Background: MAG Function and Localization

• MAG (Myelin-associated glycoprotein, also known as Siglec-4a) is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily and sialic acid-binding Ig-like lectin (Siglec) family.
• Functions as an adhesion molecule mediating interactions between myelinating cells (oligodendrocytes and Schwann cells) and neurons via binding to sialic acid-containing gangliosides and the receptors RTN4R and RTN4RL2.
• While not required for initial myelination, MAG is essential for the maintenance of normal axon myelination and prevents axonal degeneration in adults.
• Provides neuroprotection by inhibiting apoptosis of motoneurons, including after injury; this effect is likely mediated through interaction with neuronal RTN4R and RTN4RL2.
• Acts as a negative regulator of neurite outgrowth, inhibiting axon extension and longitudinal growth by binding to RTN4R and gangliosides on dorsal root ganglion and cerebellar granule cells.
• Localizes to the cell membrane, particularly in membrane rafts, where it likely facilitates signaling and adhesion.
• Both isoform 1 and isoform 2 are detected in myelinated structures of the central and peripheral nervous system, including periaxonal myelin, Schmidt-Lanterman incisures, optic nerve, and oligodendroglia, with expression confirmed in compact myelin. Related references: PMID:6200494, PMID:9495552
• Preferentially binds to α-2,3-linked sialic acid and ganglioside Gt1b, which may influence its role in axon–glia communication.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100) of human MAG, which lies within the extracellular domain. This design may enable detection of both isoforms; however, users should validate isoform specificity in their assay system.
• For Western blot, the predicted molecular weight of MAG is approximately 69 kDa, but due to glycosylation and other post-translational modifications, the observed band may shift. Use relevant positive control tissues such as brain or optic nerve, where MAG is enriched.
• In ELISA assays, the peptide immunogen may be suitable for capturing or detecting native MAG, but consider verifying performance with full-length protein or cell-based systems to ensure accurate quantification.
• Given reported cross-reactivity with mouse and rat, sequence alignment suggests high homology across these species; nonetheless, confirm cross-reactivity with appropriate controls in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing comprehensive IVD raw material solutions, connecting diagnostic manufacturers and research laboratories with premium antibodies like this anti-MAG monoclonal antibody. Whether you need validated antibody pairs, bulk ancillary reagents, or rare-target sourcing assistance, our team supports every stage from concept to clinical application. Explore our portfolio to accelerate your neuroscience and myelination research with consistent, high-quality reagents.