Anti-MAFK Rabbit Polyclonal Antibody for WB, ELISA - O60675

Core Product Specifications and Parameters

Biological Background: MAFK Function and Localization

• Transcription factor MafK (NF-E2 p18) is a member of the small Maf protein family, which lacks a transactivation domain. When small Mafs homodimerize, they act as transcriptional repressors, but they can become activators by heterodimerizing with larger bZIP proteins such as NFE2, NRF1, NRF2, and NRF3, recruiting them to specific DNA sites.
  Related references: PMID:9150357 PMID:8932385
• MAFK can heterodimerize with Fos and may serve as a competitive repressor of the NF-E2 transcription factor.
  Related references: PMID:9150357
• The protein is localized to the nucleus, consistent with its role in transcription regulation.
• Post-translational modifications include phosphorylation and isopeptide bond formation (ubiquitination), which may modulate its activity.
• Classified under keywords: DNA-binding, isopeptide bond, nucleus, phosphoprotein, repressor, transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-50) of human MAFK. This epitope is expected to detect full-length MAFK in Western blotting, with a predicted band size around 18 kDa. Consider validating detection in relevant human cell or tissue lysates.
• For ELISA, use the antibody as a capture or detection reagent depending on the assay format; optimization of coating and detection conditions may be required.
• Cross-reactivity is reported for human; reactivity with other species has not been determined; verify if needed for your experimental system.
• Since MAFK functions as part of dimeric complexes, immunoprecipitation may be possible, but it has not been validated; if attempting, consider optimizing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, including this anti-MAFK antibody for studying transcription regulation. Our comprehensive portfolio includes validated antibody pairs, bulk reagents, and sourcing support for rare targets, streamlining your development from concept to clinic. Leverage CamelBio's expertise to accelerate your MAFK-related diagnostic and research projects.