Anti-MAFF Rabbit Polyclonal Antibody for WB and ELISA - Q9ULX9

Core Product Specifications and Parameters

Biological Background: MAFF Function and Localization

• MAFF encodes transcription factor MafF, a member of the small Maf protein family lacking a putative transactivation domain.
• Small Maf proteins, including MafF, can act as transcriptional repressors when forming homodimers. Related references: PMID:8932385
• In contrast, MafF functions as a transcriptional activator upon heterodimerization with larger basic-zipper proteins such as NFE2L1/NRF1, recruiting them to specific DNA-binding sites.
• MafF binds to the upstream promoter region of the oxytocin receptor gene, contributing to its transcriptional regulation. Related references: PMID:16549056, PMID:8932385
• It may act as a transcriptional enhancer in the up-regulation of the oxytocin receptor gene during parturition. Related references: PMID:10527846
• Subcellular localization is primarily nuclear, consistent with its role as a transcription factor.
• Tissue expression reported in term myometrium and kidney.
• Keywords indicate involvement in stress response and alternative splicing, suggesting additional regulatory layers.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 75–164, an internal region of human MAFF; consider confirming reactivity with full-length and variant isoforms in your system.
• For Western blot, the predicted molecular weight is 18 kDa; however, observed bands may shift due to post-translational modifications—validate with appropriate positive controls (e.g., myometrium or kidney lysates).
• For ELISA applications, pairing with a detection antibody targeting a distinct epitope may improve sensitivity; optimize working concentrations empirically.
• Since cross-reactivity is limited to human, ensure sample species compatibility before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, supporting research from concept to clinic. This anti-MAFF polyclonal antibody is part of our transcription regulation portfolio; we also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to discuss your specific procurement needs.