Anti-MAFA Polyclonal Antibody for WB and ELISA - Q8NHW3

Core Product Specifications and Parameters

Biological Background: MAFA Function and Localization

• Transcription factor MafA (hMafA) is a pancreatic beta-cell-specific transcriptional activator and a member of the large Maf family.
• Activates insulin gene expression by binding to regulatory elements in the insulin promoter. Related references: PMID:12011435 PMID:15993959
• Binds specifically to the insulin enhancer C1/RIPE3b element. Related references: PMID:12011435
• Functions synergistically with NEUROD1/BETA2 and PDX1 to enhance insulin promoter activity. Related references: PMID:15993959
• Recognizes the consensus TRE-type MARE DNA sequence 5'-TGCTGACTCAGCA-3'. Related references: PMID:23148532 PMID:29339498
• Localizes to the nucleus, consistent with its role as a transcription factor.
• Expressed at the protein level in the islets of Langerhans, underscoring its importance in pancreatic beta cell function.
• Post-translational modifications include phosphorylation and ubiquitination; disease variants are linked to diabetes mellitus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 269-353) of human MAFA; consider this when designing detection strategies for full-length vs. truncated protein forms.
• For Western blot, a band near 37 kDa is expected; include pancreatic islet cell lysates as positive controls and validate specificity in your sample system.
• Cross-reactivity with mouse MAFA is reported, making this antibody suitable for mouse pancreatic studies; verify performance in your mouse model of interest.
• In ELISA applications, the antibody may detect both native and recombinant MAFA; consider optimizing coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and researchers developing diabetes diagnostic tools. In addition to this anti-MAFA antibody, we supply validated antibody pairs, bulk ancillary reagents, and customized raw-material procurement for rare targets. Accelerate your assay development with CamelBio's end-to-end support from concept to clinic.