Anti-MAF1 Rabbit Polyclonal Antibody for WB, ELISA - Q9H063

Core Product Specifications and Parameters

Biological Background: MAF1 Function and Localization

• MAF1 (Repressor of RNA polymerase III transcription MAF1 homolog) acts as a key repressor of RNA polymerase III, controlling the production of tRNAs, 5S rRNA, and other small non-coding RNAs to match cell growth and maintenance demands (PubMed:18377933, PubMed:20233713, PubMed:20516213). Related references: PMID:18377933, PMID:20233713, PMID:20516213

• It is also implicated in cell fate determination, promoting mesoderm induction and adipocyte differentiation (By similarity).

• Mechanistically, MAF1 associates with the RNA polymerase III clamp, impairing its recruitment to the promoter complex, thereby downregulating transcription (PubMed:17505538, PubMed:20887893). Related references: PMID:17505538, PMID:20887893

• The activity of MAF1 is tightly regulated by nutrient and stress signals via mTOR kinase: under favorable conditions, MAF1 is hyperphosphorylated and inactive; stress-induced dephosphorylation promotes its nuclear localization and repression of RNA polymerase III (PubMed:26941251). Related references: PMID:26941251

• Beyond Pol III, MAF1 may also influence RNA polymerase I and II transcription by modulating the expression of the central initiation factor TBP (PubMed:17499043). Related references: PMID:17499043

• MAF1 localizes to both the nucleus and cytoplasm, and undergoes post-translational modifications including phosphorylation, ubiquitination (isopeptide bond), and others as indicated by keywords: 3D-structure, Isopeptide bond, Phosphoprotein, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-256) of human MAF1; the resulting rabbit polyclonal antibody may recognize multiple epitopes, providing robust detection in Western blot (WB) and ELISA.
• For WB, a band near 29 kDa is expected (predicted molecular weight 29 kDa; UniProt calculated 28.8 kDa). Consider using a positive control lysate from human or mouse cells known to express MAF1.
• Due to the polyclonal nature, optimal dilutions for WB and ELISA should be determined empirically for each experimental system; cross-reactivity with mouse is reported, enabling multi-species studies.
• MAF1 expression and phosphorylation status can change under different stress or nutrient conditions; thus, sample preparation and handling may impact detection results. Validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this MAF1 Rabbit pAb, and bulk ancillary reagents. Whether you need repressor of RNA polymerase III transcription targets or rare raw materials for cell growth and stress response studies, our comprehensive sourcing services streamline your workflow from concept to clinic.