Anti-MAdCAM-1 Polyclonal Antibody for WB, ELISA - Q61826

Core Product Specifications and Parameters

Biological Background: MAdCAM-1 Function and Localization

• MAdCAM-1 (Mucosal addressin cell adhesion molecule 1) is a cell adhesion receptor expressed by mucosal venules, playing a central role in directing lymphocyte traffic into mucosal tissues such as Peyer’s patches and intestinal lamina propria.
• It binds both integrin alpha-4/beta-7 and L-selectin, thereby regulating leukocyte passage and retention within mucosal sites.
• Two isoforms exist due to alternative splicing; both adhere to integrin alpha-4/beta-7, but isoform 2 lacks the mucin-like domain and may specialize in integrin-dependent adhesion strengthening independent of L-selectin binding.
• The protein is localized to the membrane and is highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues, with lower levels in spleen, peripheral lymph nodes, and lactating mammary gland.
• MAdCAM-1 is not detected in liver, kidney, lung, or normal brain, but is upregulated in chronically inflamed tissues, including inflamed pancreas and brain endothelioma cells.
• It belongs to the immunoglobulin superfamily and undergoes post-translational modifications such as glycosylation and disulfide bond formation, contributing to its adhesion functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22–109 of mouse MAdCAM-1, covering part of the extracellular domain; epitope may be exposed in native or denatured form.
• For Western blot, consider using protein extracts from mouse intestinal lymphoid tissues or spleen where MAdCAM-1 is enriched; a band near 43 kDa is expected.
• Because the antibody is polyclonal and multiple isoforms exist, additional bands of different sizes may be detected; validate isoform specificity in your sample system.
• For ELISA, recombinant MAdCAM-1 protein or tissue lysates can be used; include appropriate controls to confirm binding specificity.
• Optimize primary antibody dilution based on the detection system and sample abundance; typical starting range for WB is 1:500–1:2000.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-MAdCAM-1 polyclonal antibody supports research into lymphocyte trafficking and mucosal immunity, with relevance to inflammatory bowel disease and autoimmune disorders. Beyond antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.