Anti-MAD3/MXD3 Rabbit Monoclonal Antibody for WB, ELISA - Q9BW11

Core Product Specifications and Parameters

Biological Background: MAD3/MXD3 Function and Localization

• MAD3/MXD3 (Max dimerization protein 3) is a transcriptional repressor that forms a sequence-specific DNA-binding complex with MAX, recognizing the E-box motif 5'-CAC[GA]TG-3', thereby antagonizing MYC-dependent transcriptional activation and cell transformation.
• The protein belongs to the basic helix-loop-helix (bHLH) transcription factor family and is also known as Class C basic helix-loop-helix protein 13 (bHLHc13), Max-associated protein 3, and Myx.
• It functions as a negative regulator of MYC oncoproteins by competing for the common binding partner MAX, thereby suppressing MYC-driven cellular proliferation and oncogenesis.
• Localized exclusively in the nucleus, consistent with its role as a DNA-binding transcriptional regulator.
• The gene MXD3 undergoes alternative splicing, producing multiple transcript variants that may expand its regulatory repertoire.
• Key post-translational modifications and interaction networks involve proteomics identification, and the protein is part of the reference proteome.
• With a calculated molecular weight of approximately 23 kDa, it is classified under keywords including DNA-binding, repressor, transcription, and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-190 of human MAD3/MXD3, within the bHLH domain; consider using full-length or domain-specific recombinant proteins as positive controls in both WB and ELISA.
• For Western blot, a single band at ~23 kDa is expected in human cell lysates; validate in your specific sample type and experimental conditions.
• ELISA applications may require titration of the antibody and antigen coating concentration; pair with a suitable secondary antibody and detection system for optimal signal.
• Cross-reactivity has been shown for human; for other species, validate experimentally as sequence homology may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For transcription regulation and cancer research, our portfolio includes validated anti-MXD3 antibodies and related reagents. Explore our full range of optimized monoclonal/polyclonal antibodies, antibody pairs, and bulk ancillary supplies to support your assay development and biomarker studies.