Anti-MAD2/MAD2L1 Rabbit Polyclonal Antibody for WB, ELISA - Q13257

Core Product Specifications and Parameters

Biological Background: Mitotic Spindle Assembly Checkpoint Protein MAD2A Function and Localization

• MAD2L1 (MAD2A) is a crucial component of the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex/cyclosome (APC/C) to delay chromosome segregation until all chromosomes are correctly attached to the mitotic spindle.
  Related references:
  PMID:10700282
  PMID:11804586
  PMID:15024386
• The MCC is composed of CDC20, MAD2L1, BUB1B/BUBR1, and BUB3; its binding to APC/C maintains the complex in an inactive state, preventing premature anaphase onset.
  Related references:
  PMID:15020684
  PMID:27509861
• Upon silencing of the spindle assembly checkpoint, the MCC-APC/C complex recruits the E2 enzyme UBCH10, leading to auto-ubiquitination and degradation of CDC20. This reactivates APC/C, driving the metaphase/anaphase transition.
  Related references:
  PMID:27509861
• MAD2L1 adopts two distinct conformations: open (O-MAD2) and closed (C-MAD2). The closed form assembles with MAD1L1 at unattached kinetochores to create a template that catalyzes the conversion of O-MAD2 to C-MAD2, amplifying the checkpoint signal.
  Related references:
  PMID:29162720
• Alternative splicing produces two isoforms (MAD2A and MAD2B); MAD2A (encoded by MAD2L1) is the mitotically regulated isoform essential for spindle checkpoint function.
• The protein localizes dynamically throughout the cell cycle: it resides in the cytoplasm and nucleus during interphase, concentrates at kinetochores and spindle poles during mitosis, and relocalizes upon checkpoint activation.
• Post-translational modifications, including acetylation and phosphorylation, fine-tune MAD2L1 activity and interactions. Its UniProt-calculated molecular weight is ~23.5 kDa, while product data indicate ~24 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-205) encompasses the N-terminal region critical for MAD1 interaction and conformational switching. This design may enable detection of both O-MAD2 and C-MAD2 forms.
• For Western blot, anticipate a band near 24 kDa. Use mitotically synchronized cells (e.g., nocodazole-treated) alongside interphase controls to confirm specificity.
• ELISA applications can leverage direct or sandwich formats; consider using recombinant MAD2L1 protein as standard.
• The antibody is predicted to cross-react with mouse and rat MAD2L1 based on sequence conservation; validation in appropriate tissue or cell lysates is recommended.
• For functional studies, combine with cell synchronization protocols to enrich mitotic populations and enhance signal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials and technical support. This anti-MAD2L1 polyclonal antibody supports cell cycle and oncology research; developers can also source validated antibody pairs, recombinant proteins, and ancillary reagents through CamelBio’s integrated supply network. Whether you need rare-target antibodies or optimization of detection systems, CamelBio offers tailored B2B solutions to accelerate your project from concept to clinic.