Anti-MAD2/MAD2L1 Rabbit Monoclonal Antibody for WB, ELISA - Q13257

Core Product Specifications and Parameters

Biological Background: MAD2/MAD2L1 Function and Localization

• MAD2/MAD2L1 (Mitotic arrest deficient 2-like protein 1), also known as MAD2A or HSMAD2, is a key component of the mitotic checkpoint complex (MCC) that ensures accurate chromosome segregation during cell division. Related references: PMID:10700282, PMID:11804586, PMID:15024386
• The MCC, comprising MAD2L1, CDC20, BUB1B/BUBR1, and BUB3, binds to and inhibits the anaphase-promoting complex/cyclosome (APC/C), delaying the metaphase-to-anaphase transition until all chromosomes are properly attached to the mitotic spindle. Related references: PMID:15020684, PMID:27509861
• Upon satisfaction of the spindle assembly checkpoint, the MCC-APC/C complex recruits the E2 enzyme UBCH10, triggering auto-ubiquitination and degradation of CDC20, which reactivates APC/C for anaphase onset. Related references: PMID:27509861
• MAD2L1 exists in two conformational states: open (O-MAD2) and closed (C-MAD2). At unattached kinetochores, C-MAD2 forms a heterotetramer with MAD1L1, which recruits O-MAD2 molecules and catalyzes their conversion to the active C-MAD2 form. Related references: PMID:29162720
• The protein localizes to the nucleus, specifically at kinetochores on condensed chromosomes, as well as to the cytoplasm and spindle poles during mitosis.
• Keywords associated with MAD2L1 include acetylation, phosphorylation, and alternative splicing, suggesting post-translational modifications and isoform diversity that may regulate its checkpoint functions.

Experimental Guidance and Technical Tips

• The antibody was generated against a synthetic peptide spanning amino acids 70-150 of human MAD2L1. This region may be accessible in both open and closed conformations, but empirical validation in relevant sample systems is recommended.
• Suitable applications as provided include Western blotting (WB) and ELISA. For WB, consider a loading control that is not cell-cycle regulated, and optimize blocking conditions to minimize background.
• Given the dynamic localization and conformational changes of MAD2L1 during mitosis, cell synchronization techniques may enhance detection in specific cell cycle phases.

CamelBio: Your One-Stop Sourcing Bridge

As your dedicated IVD raw-material sourcing partner, CamelBio provides one-stop access to high-quality reagents for cell cycle and mitosis research. We offer validated monoclonal and polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to support diagnostic development. Whether you require routine basal antibodies or rare target sourcing, our supply chain is designed to bridge your concept-to-clinic needs.