Anti-MAD2L1BP Rabbit Monoclonal Antibody for IHC-P, IF/ICC, ELISA - Q15013

Core Product Specifications and Parameters

Biological Background: MAD2L1BP Function and Localization

• Also known as Caught by MAD2 protein or p31(comet), MAD2L1BP is encoded by the MAD2L1BP gene (alias CMT2, KIAA0110) in humans.
• Its primary function is to silence the spindle assembly checkpoint, enabling mitotic progression through anaphase by binding MAD2L1 after it dissociates from the MAD2L1-CDC20 complex.
• MAD2L1BP localizes to the nucleus, cytoplasm, and associates with the cytoskeleton and mitotic spindle, consistent with its role in chromosome segregation.
• The protein is a phosphoprotein, suggesting regulation by post-translational modification, and its 3D structure has been resolved.
• Alternative splicing generates multiple transcript variants, potentially expanding functional diversity.
• It is identified as a reference proteome member and has been detected in multiple proteomics studies, underscoring its fundamental role in cell cycle control.

Experimental Guidance and Technical Tips

• As a rabbit monoclonal antibody raised against a synthetic peptide, it may recognize a linear epitope and could perform well under both denaturing (IHC-P) and native (IF/ICC) conditions, but empirical validation in the target sample system is recommended.
• For IHC-P, consider optimizing antigen retrieval methods and using appropriate blocking agents to minimize background.
• In IF/ICC, co-staining with spindle or cytoskeletal markers may help confirm localization patterns.
• Although the antibody reacts with human and mouse samples, cross-reactivity with other species should be independently verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering everything from validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. For researchers and diagnostic developers focusing on spindle checkpoint regulation and cell cycle biology, CamelBio provides this anti-MAD2L1BP antibody alongside complementary tools to streamline your project from concept to clinic.