Anti-MAD2B/MAD2L2 Polyclonal Antibody for WB - Q9UI95

Core Product Specifications and Parameters

Biological Background: MAD2B/MAD2L2 Function and Localization

• MAD2B/MAD2L2 (also known as REV7, FANCV, MAD2B, POLZ2) is an adapter protein with diverse roles in DNA damage repair, cell cycle regulation, and transcription (PubMed:11459825, PubMed:11459826, PubMed:17296730). Related references: PMID:11459825, PMID:11459826, PMID:17296730
• It mediates translesion DNA synthesis by facilitating the interaction between REV3L (DNA polymerase zeta catalytic subunit) and REV1, enabling replication past DNA lesions (PubMed:20164194, PubMed:23143872). Related references: PMID:20164194, PMID:23143872
• As a component of the shieldin complex, MAD2B/MAD2L2 promotes non-homologous end joining (NHEJ) repair of DNA double-strand breaks and suppresses DNA end resection (PubMed:29656893). Related references: PMID:29656893
• It inhibits the anaphase-promoting complex (APC) via FZR1 and CDC20, thereby regulating mitotic progression and cell cycle checkpoints (PubMed:11459825, PubMed:17719540). Related references: PMID:11459825, PMID:17719540
• Involved in transcriptional regulation, including modulation of TCF7L2-mediated gene transcription and potential roles in epithelial-mesenchymal transition (PubMed:19443654). Related references: PMID:19443654
• Subcellularly, MAD2B/MAD2L2 localizes to the nucleus, cytoplasm, spindle, and chromosomes, consistent with its roles in mitosis and DNA repair.
• It is ubiquitously expressed across tissues, reflecting its fundamental cellular functions.
• Keywords associated with MAD2B/MAD2L2 include Cell cycle, Cell division, DNA damage, DNA repair, Fanconi anemia, Mitosis, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen used for this polyclonal antibody corresponds to a recombinant fusion protein covering amino acids 1-211 of human MAD2B/MAD2L2. This N-terminal region may be suitable for detecting both full-length and potential isoforms, but users should validate specificity in their sample system.
• For western blotting, the predicted protein weight is 24 kDa; consider appropriate gel percentages and ensure proper loading controls.
• ELISA applications may require optimization of coating antigen and antibody concentration.
• Cross-reactivity with mouse is reported, so this antibody may be useful in mouse models, but cross-reactivity with other species should be verified independently.
• Given the target's involvement in DNA damage response, consider appropriate treatments (e.g., genotoxic agents) to modulate expression or localization in functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio, as a one-stop IVD raw-material sourcing partner, offers validated antibodies like this anti-MAD2B/MAD2L2 pAb, along with monoclonal alternatives, antibody pairs, and bulk ancillary reagents. Our portfolio supports DNA damage repair and cell cycle research areas with optimized reagents and consulting services from concept to clinic.