Anti-MAD2B/MAD2L2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UI95

Core Product Specifications and Parameters

Biological Background: MAD2B/MAD2L2 Function and Localization

• Adapter protein capable of interacting with different partners and involved in DNA repair, cell cycle regulation, and transcription. Related references: PMID:11459825, PMID:11459826, PMID:17296730
• Mediates interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, facilitating the second polymerase switching in translesion DNA synthesis (TLS). Related references: PMID:20164194, PMID:23143872
• Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs) and promotes non-homologous end joining (NHEJ). Related references: PMID:29656893
• Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC, thereby regulating progression through the cell cycle. Related references: PMID:11459825, PMID:17719540
• Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. Related references: PMID:19443654
• Localizes to the nucleus, cytoplasm, cytoskeleton, spindle, and chromosomes during cell division.
• Ubiquitously expressed across tissues; implicated in Fanconi anemia pathway.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 24 kDa. Consider using whole cell lysates from human, mouse, or rat cell lines as positive controls.
• For immunofluorescence/immunocytochemistry, appropriate fixation and permeabilization protocols may be needed. Validate staining patterns in cells that express endogenous MAD2L2.
• The immunogen corresponds to the N-terminal region (aa 1-211), ensuring recognition of the full-length protein; however, cross-reactivity with other MAD2 family members has not been specified.
• For ELISA applications, recombinant MAD2L2 protein can serve as a standard. Titrate the antibody to determine optimal working concentrations.
• Given MAD2L2’s role in cell cycle and DNA damage response, consider using cell cycle synchronization or DNA-damaging agent treatments to investigate expression changes. Always include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop sourcing for IVD raw materials, including validated antibodies like this anti-MAD2B/MAD2L2 polyclonal antibody for DNA damage and cell cycle research. We supply optimized monoclonal and polyclonal antibodies, ready-to-use antibody pairs, and bulk ancillary reagents to support your diagnostic and laboratory workflows. Our expert team can also assist with sourcing rare-target raw materials, ensuring a seamless transition from concept to clinic.