Anti-MAD1/MAD1L1 Rabbit Monoclonal Antibody for WB, ELISA - Q9Y6D9

Core Product Specifications and Parameters

Biological Background: MAD1/MAD1L1 Function and Localization

• MAD1/MAD1L1 (MAD1L1 gene) encodes mitotic spindle assembly checkpoint protein MAD1, also known as HsMAD1, hMAD1, and Tax-binding protein 181.
• It is a core component of the spindle-assembly checkpoint (SAC) that delays anaphase onset until all chromosomes are properly aligned at the metaphase plate. Related references: PMID:10049595, PMID:20133940, PMID:29162720
• At unattached kinetochores, MAD1 forms a heterotetrameric complex with closed MAD2L1 (C-MAD2) and catalyzes the conversion of open MAD2L1 (O-MAD2) to C-MAD2, amplifying the “wait anaphase” signal. Related references: PMID:29162720
• MAD1 can also sequester MAD2L1 in the cytoplasm, impairing SAC function and promoting chromosomal instability, particularly in hepatocellular carcinomas.
• Subcellular localization: dynamically localizes to the nucleus, kinetochore, nuclear envelope, centrosome, mitotic spindle, and spindle poles during cell cycle progression.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitin-like conjugation (ISGylation), which may regulate its checkpoint functions.
• Expressed at the protein level in hepatocellular carcinomas and hepatoma cell lines, linking it to liver cancer biology.
• MAD1 belongs to the MAD (mitotic arrest deficient) family, essential for mitotic checkpoint control.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50–150 of human MAD1. Verify antibody specificity using peptide competition assays or genetic knockdown/knockout controls in the relevant cell system.
• Predicted molecular weight of MAD1 is ~83 kDa; a band near this size should be expected in Western blot. Consider using reducing conditions and appropriate loading controls.
• For ELISA, the antibody may be used as a capture or detection reagent; optimize coating concentration and pairing with matched antibodies for sandwich ELISA development.
• Cross-reactivity is reported for human and mouse; validate in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, supporting diagnostic manufacturers and research laboratories from early development through clinical validation. Our anti-MAD1/MAD1L1 antibody is part of a comprehensive portfolio for mitosis and oncology research, and we offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and sourcing of rare-target raw materials. For tailored procurement, technical consultation, and efficient supply chain solutions, contact CamelBio.